Affinity-mass spectrometric technologies for quantitative proteomics in biological fluids
نویسندگان
چکیده
منابع مشابه
Mass spectrometric-based approaches in quantitative proteomics.
Classically, experiments aimed at studying changes in protein expression have always followed a small set of proteins. This focused approach was necessary since tools to efficiently analyze large numbers of proteins were simply not available. Large-scale quantitative proteomics promises to produce reams of data that previously would have taken decades to measure with classical methods. Mass spe...
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In the late 1980s, the advent of soft ionization techniques capable of generating stable gas phase ions from thermally unstable biomolecules, namely matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI), laid the way for the development of a set of powerful alternatives to the traditional Edman chemistry for the structural characterization of peptides and protein...
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Research of new diagnosis or prognosis biomarkers is a major challenge for the management of patients with complex pathologies like cancer. Clinical proteomics is one of the recent approaches to identify these biomarkers in biological fluids. Over the last five years, many problems related to the variability and the quality control of these analyses have been observed. This was notably related ...
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ژورنال
عنوان ژورنال: TrAC Trends in Analytical Chemistry
سال: 2017
ISSN: 0165-9936
DOI: 10.1016/j.trac.2017.02.011